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To measure complex-specific H 2 O 2 production, the cells were incubated with 10 mM pyruvate/1 mM malate, 10 mM succinate/2 M rotenone, or 5 mM glycrol-3-phosphate/2 M rotenone diluted in MAS buffer containing 4 mM ADP and 1 nM XF PMP for 30 min at 37 C prior to the addition of Amplex Red